Genetic question need help! Consider an E. coli partial diploid strain; on its chromosome.....?

Posted on 2016-03-16 18:42:00 in Biology by YUKI

Consider an E. coli partial diploid strain; on its chromosome, it is LacI+ and lacO+Z-Y+ and on the F’ it carries lacI+, lacOcZ+Y-. In this strain, lacY activity will be 

A. induced in the absence of lactose and induced in the presence of lactose
B. uninduced in the absence of lactose and induced in the presence of lactose
C. uninduced in the absence of lactose and uninduced in the presence of lactose
D. induced in the absence of lactose and uninduced in the presence of lactose
E. None of the above 

When genes are introduced into plant nuclei using Agrobacterium tumefaciens, the T-DNA is: 

A. Maintained as a linear peiece of DNA in the plant nucleus
B. Always recombined at a homologous site in the plant chromosome
C. Integrated at a random position in one of the plant’s chromosomes
D. Maintained as a circular plasmid in the plant nucleus
E. None of the above 

In the ‘A’ reaction in Sanger, chain-terminator sequencing, when DNA polymerase reaches a T in the template, it… 

A. Always incorporates ddATP
B. Can incorporate either dATP or ddATP
C. Can only incorporate dATP
D. Can only incorporate dTTP
E. Can incorporate either dTTP or ddGTP 


A lac- mutant that is unable to transport lactose into the cell would likely carry a mutation in the 


A. lacZ gene 
B. lacY gene
C. LacA gene
D. lacO gene 
E. none of the above

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